Plasmid
pTG262

Part:BBa_I742103:Design

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-06)


pTG262 Plasmid (Gram negative to Gram positive shuttle vector)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 463
    Illegal XbaI site found at 436
    Illegal PstI site found at 424
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 463
    Illegal NheI site found at 5313
    Illegal PstI site found at 424
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 463
    Illegal BglII site found at 2252
    Illegal BamHI site found at 442
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 463
    Illegal XbaI site found at 436
    Illegal PstI site found at 424
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 463
    Illegal XbaI site found at 436
    Illegal PstI site found at 424
    Illegal AgeI site found at 2641
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2427


Design Notes

Plasmid had to be able to act as a shuttle vector between E. coli and Lactobacillus, replicated in both organisms and have suitable resistance genes and stability


Source

Kindly donated by Dr Mike Gasson & Dr. Claire Shearman, of the Institute of Food Research in Norwich, United Kingdom

References